Three-Dimensional Model of Normal Human Dermal Tissue Using Serial Tissue Sections

This study introduces a new method for creating high-resolution 3D models of human dermal tissue using serial tissue sections, which surpasses traditional methods in accuracy. By analyzing skin samples from normal adults, the research identified that dermal fibers are interwoven, forming pores with varying sizes and fibroblast content. The study quantified dermal porosity at 18.96% ± 4.41% and average pore diameter at 219.29 ± 34.27 μm, noting variations across skin layers. These findings are crucial for designing biomimetic tissue-engineered skin, which could improve wound healing and minimize scarring. The study also highlights the importance of precise image alignment, employing the SIFT algorithm to enhance data accuracy, thus providing a valuable dataset for future skin scaffold development.