4-Aminopyridine Promotes Accelerated Skin Wound Healing via Neurogenic-Mediator Expression

March 2022 in “ Research Square (Research Square) ”

Mashanipalya Jagadeeshaprasad, Prem Kumar Govindappa, Amanda M. Nelson, Mark David Noble, John C. Elfar

4-aminopyridine potassium channel blocker skin wound healing hair follicle neogenesis vimentin alpha-smooth muscle actin dermis neuronal regeneration axons S100+ Schwann cells SRY-Box Transcription Factor 10 transforming growth factor-β substance P nerve growth factor human keratinocytes Schwann cells 4-AP TGF-β NGF

TLDR 4-aminopyridine, a FDA-approved drug, speeds up skin wound healing and tissue regeneration.

The document reveals that 4-aminopyridine (4-AP), a potassium channel blocker approved by the FDA, significantly improves skin wound healing. This includes faster wound closure, restoration of normal skin structure, increased vascularization and reinnervation, and enhanced hair follicle neogenesis. The study found that 4-AP increased levels of vimentin and alpha-smooth muscle actin in the healed dermis, contributing to tissue regeneration. It also promoted neuronal regeneration, as evidenced by increased numbers of axons and S100+ Schwann cells, and elevated expression of SRY-Box Transcription Factor 10. The treatment also boosted levels of transforming growth factor-β, substance P, and nerve growth factor, all crucial for wound healing. In-vitro studies showed that 4-AP enhanced the proliferation and migration of human keratinocytes and Schwann cells, and improved cellular interactions to accelerate wound healing. Therefore, 4-AP enhances many key aspects of successful wound healing and presents a promising new approach to improve skin wound healing and tissue regeneration.

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