Bioactive Substances Derived From Probiotic Lactobacillus acidophilus Reduce Motility And Viability In Cervical Cancer Cells

The study investigates the anticancer potential of Lactobacillus acidophilus cell-free supernatant (LACFS) against human cervical cancer cell lines, SiHa and C33A. The results show that LACFS significantly reduces cell viability in a concentration-dependent manner, with SiHa cells being more sensitive. Morphological changes indicative of apoptosis, such as cell shrinkage and membrane blebbing, were observed. Additionally, LACFS impaired the migratory ability of the cancer cells and increased both early and late apoptotic cells, with minimal necrosis. These findings suggest that L. acidophilus metabolites have strong cytotoxic, anti-migratory, and apoptosis-inducing effects, highlighting their potential as a safe and cost-effective adjunct therapy for cervical cancer.