Biotin Supplementation Ameliorates Murine Colitis by Preventing NF-κB Activation

The study explored the effects of biotin supplementation on colitis in mice, finding that high-dose biotin delayed the onset and severity of colitis and accelerated healing. Mice treated with biotin showed improvements in the Disease Activity Index, fecal calprotectin levels, colon length, and histology. Biotin reduced the expression of inflammatory cytokines, intestinal permeability, and activation of NF-κB, a transcription factor involved in inflammation. These findings suggested that biotin could be a potential therapeutic for inflammatory bowel disease (IBD) by maintaining intestinal barrier integrity and reducing inflammation, highlighting the clinical relevance of the biotin transport pathway as a target for IBD treatment.