Mechanism of Cannabinoid Incorporation in Hair

The dissertation investigated the mechanism of cannabinoid incorporation into hair, specifically focusing on delta-9-tetrahydrocannabinol (THC) and 11-nor-delta-9-tetrahydrocannabinol (THCA). It was hypothesized that these cannabinoids incorporate into hair independently of melanin binding, unlike some basic drugs. Experiments with pigmented and nonpigmented hair from Long-Evans rats showed no significant difference in THC and THCA incorporation, supporting the hypothesis. Further studies using cultured melanocytes indicated that factors other than pigmentation affect cannabinoid retention. THC was found to localize with epidermal keratins and sebaceous glands after multiple administrations, suggesting incorporation via sebum and skin rather than direct melanin interaction.