Short-Term Cryoprotectant-Free Cryopreservation at −20°C Does Not Affect the Viability and Regenerative Capacity of Nanofat
July 2024
in “
Frontiers in Bioengineering and Biotechnology
”
TLDR Storing nanofat at -20°C for 7 days does not harm its ability to regenerate.
The study concludes that nanofat can be effectively cryopreserved at −20°C without cryoprotectants for short periods, maintaining its viability and regenerative capacity. This was demonstrated through comparable vascularization, tissue formation, and extracellular matrix composition in wounds treated with either fresh or cryopreserved nanofat. The study involved 8 samples per group and found no significant differences in microvessel and lymph vessel densities, as well as macrophage ratios, between the two groups, suggesting this method is viable for clinical applications.
