Efficient expansion of human keratinocyte stem/progenitor cells carrying a transgene with lentiviral vector
October 2013
in “
Stem Cell Research & Therapy
”
TLDR A specific combination of chemicals effectively expands genetically modified skin cells for potential use in skin therapy.
The study investigated the efficient expansion of human keratinocyte stem/progenitor cells carrying a transgene using a lentiviral vector. It found that polybrene enhanced lentiviral gene transduction but negatively impacted cell maintenance at concentrations above 5 μg/ml, while the ROCK inhibitor Y-27632 promoted proliferation but interfered with transduction. An optimal combination of 2.5 μg/ml polybrene and Y-27632 effectively expanded keratinocytes carrying a transgene, providing insights for regenerative medicine applications. The research highlighted the potential of genetically modified keratinocyte stem/progenitor cells in creating functional epidermal sheets for cell therapy in genetic skin disorders.
