Fabrication of Bilayer Nanofibrous-Hydrogel Scaffold from <i>Bacterial Cellulose</i>, <i>PVA</i>, and <i>Gelatin</i> as Advanced Dressing for Wound Healing and Soft Tissue Engineering

The study introduces a bilayer nanofibrous-hydrogel scaffold made from bacterial cellulose, PVA, and gelatin, designed for wound healing and soft tissue engineering. The scaffold features a top layer of electrospun PVA-containing bacterial cellulose nanofibers and a bottom layer of a porous gelatin-PVA hydrogel. It exhibits hydrophilicity, high swelling capacity, and stability, with effective antibacterial activity due to sustained Ag-sulfadiazine release. In vitro tests on 3T3 and HEK-293 cell lines showed excellent hemocompatibility, cell viability, proliferation, and adhesion, indicating its potential as an advanced wound dressing material. The scaffold's properties, such as biocompatibility, mechanical strength, and moisture retention, make it a promising material for wound dressings and tissue engineering applications.