FLIM Reveals Red Light-Induced Changes in Murine Hair Follicles

This study investigates the effects of red-light irradiation on hair follicle dynamics using fluorescence lifetime imaging microscopy (FLIM) in a C57BL/6 mouse model. The research demonstrates that 650 nm red-light irradiation significantly increases hair follicle numbers and enhances ATP levels in skin tissue. FLIM analysis reveals prolonged fluorescence lifetime values, indicating significant changes in the skin's metabolic microenvironment. The study highlights FLIM's high sensitivity and accuracy in evaluating hair growth, suggesting its potential as a powerful tool for characterizing the cutaneous microenvironment and assessing phototherapeutic efficacy in future applications.