Flow Cytometry in the Differential Diagnosis of Neutropenic Patients
June 2023
in “
Zenodo (CERN European Organization for Nuclear Research)
”
TLDR Neutropenic patients show significant changes in immune cell types and lower neutrophil and natural killer cell percentages.
This study investigated the expressions of lymphocyte, granulocyte, and monocyte subtypes in 24 patients with isolated neutropenia compared to 13 healthy volunteers using flow cytometry. Key findings include a higher neutrophil/lymphocyte (N/L) mean fluorescence intensity (MFI) ratio in patients, a positive correlation between leukocyte counts and atypical monocytes expressing CD16, and a negative correlation between platelet counts and both the N/L MFI ratio and CD11b expression on neutrophils. Additionally, the percentage of neutrophils and natural killer cells was significantly lower in the patient group. These results indicate significant changes in lymphocyte and monocyte subtypes in neutropenic patients.
