<i>In vivo</i> assessment of mechanical properties during axolotl development and regeneration using confocal Brillouin microscopy

The study utilized confocal Brillouin microscopy to assess mechanical properties in vivo during axolotl development and regeneration. This non-invasive technique allowed for high-resolution mapping of the Brillouin frequency shift in developing limbs and regenerating digits, revealing a gradual increase in cartilage Brillouin frequency shift. This suggested a decrease in tissue compressibility during both development and regeneration, which correlated with the regeneration stage and was not detectable with fluorescence microscopy. The findings highlighted the potential of Brillouin microscopy to explore mechanical changes in axolotls, contributing to the field of epimorphic regeneration.