Development of an In Vitro Inhalation Method for Characterization of Aerosols Released During the Use of Hair Products

The study developed an in vitro inhalation method to assess the respiratory risks of aerosols from hair products, particularly for hairdressers. Using human lung cells (A549), the study evaluated four test materials and different application techniques. While cell viability and mitochondrial function were largely unaffected, differences in cellular stress and IL-8 release were observed. A scoring system was created to assess aerosol release and biological effects, showing promising alignment with real-life observations. This method offers a potential alternative to animal testing for evaluating the respiratory effects of cosmetic aerosols and could aid in developing safer products.