Probing Keratinocyte and Differentiation Specificity of the Human K5 Promoter In Vitro and in Transgenic Mice

    June 1993 in “ Molecular and Cellular Biology ”
    C Byrne, Elaine Fuchs
    TLDR The K5 promoter controls gene expression in skin cells, with specific DNA segments crucial for targeting and regulation.
    The study investigated the regulatory mechanisms of the human K5 gene expression in keratinocytes, both in vitro and in transgenic mice. It was found that 6,000 base pairs of the K5 promoter directed specific expression in basal cells of stratified epithelia, while a truncated 90 bp promoter still targeted expression to stratified epithelia, particularly in the epidermis, hair follicles, and tongue. However, this truncated promoter showed some deviations in expression patterns. Protein-DNA interaction studies revealed that keratinocyte nuclear proteins binding to the K5 gene segment from -90 to +32 bp played a role in transcriptional regulation. These proteins were shared with the K14 promoter but differed from previously identified keratinocyte-specific factors. Mutagenesis indicated that factors near the TATA box and transcription initiation site were crucial for the cell type specificity of the truncated promoter.
    Discuss this study in the Community →

    Related Community Posts Join

    6 / 43 results

      community Minoxidil is NOT an Anti-androgen. No.

      in Research/Science  43 upvotes 2 months ago
      Minoxidil is not an anti-androgen; it is a growth stimulator and does not lower DHT like finasteride or dutasteride. Minoxidil can cause side effects like facial swelling and anxiety due to its vasodilator properties.

      community Minoxidil Sulfate makes everyone a responder

      in Research/Science  260 upvotes 1 year ago
      Minoxidil sulfate is more effective than regular minoxidil, especially for those with low sulfotransferase levels or scalp sensitivity, but it is unstable unless delivered in a liposomal format. Combining minoxidil with tretinoin can enhance effectiveness, and stopping minoxidil use can lead to rapid hair loss.

      community AGA responded to steroid shots? Biopsy wrong? Please help!

      in Female  2 upvotes 1 year ago
      A woman with AGA is using spironolactone, dutasteride, finasteride, oral minoxidil, bicalutamide, and anti-androgenic birth control but still experiences worsening hair loss. Steroid shots temporarily stop her hair shedding, leading her to question her biopsy results.

      community Study on Sugar Intake on Male Pattern Hair Loss

      in Research/Science  20 upvotes 3 years ago
      Excessive sugar and high-fat diets are linked to male pattern hair loss, with sweet beverages being a significant factor. Moderation in diet is advised, as diet alone may not fully prevent hair loss due to other factors like DHT sensitivity.

      community Is Vitamin D the cause of my baldness or slow regrowth?

      in Is this regrowth?  1 upvotes 3 months ago
      The conversation discusses hair loss treatments, including oral finasteride, topical minoxidil, and microneedling, and the potential impact of Vitamin D and folic acid deficiencies on hair regrowth. It suggests that while male pattern baldness is the primary cause, addressing vitamin deficiencies may aid in improving regrowth.

    Related Research

    6 / 6 results