Single Injection of Very Mild Dose Botulinum Toxin in the Vastus Lateralis Improves Testicular Spermatogenesis and Sperm Motility in Aging Experimental Mice

The study examined the impact of a single, mild dose of botulinum toxin (BoNT) injected into the vastus lateralis muscle on testicular function in 12 ageing male BALB/c mice. The results showed that BoNT improved spermatogenesis and sperm motility, increased total sperm count, and reduced morphological defects. Histological analysis revealed enhanced spermiogenesis, and BoNT treatment boosted key testicular antioxidants, potentially mitigating oxidative stress. The findings suggested that BoNT could counteract ageing-associated declines in male reproductive function, offering a potential therapeutic strategy for male infertility, though further research would be necessary to assess its applicability to humans.