Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry
September 2023
in “
Molecules
”
TLDR These methods help understand cell structures and reactions.
The review explores the use of Electron Paramagnetic Resonance (EPR), Raman Spectroscopy, and Differential Scanning Calorimetry (DSC) in studying molecular compounds involved in cellular metabolic processes. EPR is noted for detecting free radicals and assessing oxidative stress, while Raman Spectroscopy, especially with surface-enhanced techniques, aids in classifying bacterial strains and addressing drug resistance. DSC is used to study lipid-centered drug therapy and antioxidant activity. These methods provide complementary insights into molecular mechanisms, with applications in antioxidant activity, oxidative stress, and drug delivery systems. The review also highlights the development of a semiempirical model for determining antioxidant capacity, emphasizing the importance of understanding complex molecular processes in cell envelopes, particularly in drug-resistant strains of M. tuberculosis.
