Non-Healing Wound Model in Diabetic C57BL/6 Mice

The study developed a non-healing diabetic wound model in C57BL/6 mice, involving 32 mice divided into diabetic and control groups. Diabetes was induced using streptozotocin, and wounds were created to assess healing dynamics. Results showed that diabetic mice experienced significant delays in wound healing, characterized by persistent inflammation, delayed re-epithelialization, and impaired tissue maturation compared to controls. Despite similar overall wound closure rates by day 15, diabetic mice exhibited chronic wound characteristics, including excessive neutrophil infiltration and disrupted angiogenesis. The model effectively mimics human diabetic wound mechanisms, making it suitable for preclinical testing of therapeutic interventions.