Isolation, Culture, And Potential Use Of Porcine Neural And Epidermal Stem Cells

December 2004 in “ Reproduction Fertility and Development ”

Jan Motlík, Petr Vodička, Jiřı́ Klı́ma, Karel Smetana, F. Liu, Hans‐Joachim Gabius

epidermal stem cells hair follicles keratinocytes galectin-1 ΔNp63α

TLDR Porcine hair follicles are useful for studying keratinocyte function, with galectin-1 as a potential stem cell marker.

This study focused on the isolation and culture of porcine neural and epidermal stem cells, using miniature pigs as a biomedical model. Researchers isolated multipotent cells from the brains of 40- and 80-day-old porcine fetuses, which were cultured to form floating spheres containing undifferentiated and differentiated cells, including neurons and glia. Epidermal stem cells (EpSC) were characterized by their slow proliferation rate and were primarily located in the bulge region of hair follicles. A new method was developed to isolate porcine hair follicles, and keratinocytes from these follicles expressed specific markers like galectin-1. The study concluded that porcine hair follicles are a valuable model for studying hair follicle-originated keratinocytes, with galectin-1 as a potential marker for the stem cell compartment in vitro.

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