Protocol for Preparing Mammalian Skin Samples Encompassing Hair Follicles for Spatial Transcriptomics

This document outlines a protocol for preparing mammalian skin samples, including hair follicles, for spatial transcriptomics, which allows for single-cell resolution analysis of gene expression. The protocol includes detailed steps for sample preparation, embedding, freezing, RNA quality control, tissue mounting, fixation, staining, imaging, permeabilization, reverse transcription, and cDNA collection. This method addresses the challenges of applying spatial transcriptomics to skin tissues, providing a comprehensive guide for researchers in the field.