TLDR Fibroblasts from healthy donors can prevent changes seen in recessive epidermolysis bullosa simplex.
The study successfully reproduced the basal cell vacuolization characteristic of recessive epidermolysis bullosa simplex (REBS) in vitro using fibroblasts and skin biopsies from a REBS patient and a healthy donor, but it was unable to replicate the epidermolytic hyperkeratosis (EHK) phenotype. It highlighted the significant role of fibroblasts in influencing the REBS phenotype, suggesting that wild-type fibroblasts might stabilize K14−/− keratinocytes in vitro. The study involved culturing skin biopsies on collagen matrices populated with different fibroblast types for 32 days, revealing that fibroblasts from healthy donors prevented the morphological changes seen in REBS. The absence of K14 in REBS patients was compensated by up-regulation of K15, and the study concluded that K14 ablation effects could be replicated in vitro, offering a model for studying genetic disorders' mechanisms.
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