Histological and Molecular Restoration of Type VII Collagen in Recessive Dystrophic Epidermolysis Bullosa Mouse Skin by Topical Injection of Keratinocyte-Like Cells Differentiated from Human Adipose-Derived Mesenchymal Stromal Cells

The study explores the use of keratinocyte-like cells derived from human adipose-derived mesenchymal stromal cells (KC-AD-MSCs) to treat Recessive Dystrophic Epidermolysis Bullosa (RDEB) in mice. By injecting these cells into the skin, researchers observed the deposition of type VII collagen at the dermal-epidermal junction, which is essential for skin integrity. This treatment reduced blister formation and improved skin structure, indicating that KC-AD-MSCs could potentially correct collagen insufficiency and repair anchoring fibrils in RDEB. Although the number of anchoring fibrils was not fully restored, it was adequate to maintain function. The study suggests KC-AD-MSCs as a promising treatment for RDEB, but further research is needed to refine cell dosage and administration.