Advancing Skin Model Development: A Focus on a Self-Assembled, Induced Pluripotent Stem Cell-Derived, Xeno-Free Approach

The study introduces a self-assembled skin model (SASM) using induced pluripotent stem cells (iPSC) derived from hair follicle-derived keratinocytes, developed in a xeno-free environment. This model successfully forms distinct epidermal and dermal layers, with iPSC-derived fibroblasts secreting collagen to create the dermis, and keratinocytes forming a stratified epidermis. The SASM demonstrates functional integrity by responding to sensitizing agents, indicating its potential for dermatological research. The approach addresses ethical concerns by eliminating animal-derived components and offers a reliable alternative for skin tissue engineering, aligning with efforts to reduce animal testing. The study highlights the role of L-ascorbic acid in enhancing fibroblast differentiation and collagen synthesis, underscoring the model's potential applications in regenerative medicine and therapeutic testing.