Sterilization and low temperature effects on regenerative potential of hyaluronic acid

The study investigated a sterilization method for hyaluronic acid (HA) solutions that preserves their regenerative properties, focusing on the effects of low temperatures. Tyndallization, a gentle sterilization process, was found to maintain the sterility and regenerative potential of 1% and 2% HA solutions with varying molecular weights. The research demonstrated that low temperatures, including thermocycling, do not alter the regenerative properties of both low molecular weight (LMW) and high molecular weight (HMW) HA. This finding supports the potential use of HA in cryobiology and cryomedicine, as its regenerative properties remain intact under these conditions.