Type 2 Cytokine-Dependent Skin Barrier Regulation in Personalized 2D and 3D Skin Models of Atopic Dermatitis: A Pilot Study

August 2024 in “ JID Innovations ”

Hila Emmert, Franziska Rademacher, Matthias Hübenthal, Regine Gläser, Hanne Norsgaard, Stephan Weidinger, Jürgen Harder

keratinocytes hair follicles atopic dermatitis type 2 cytokines IL-4 IL-13 epidermal spongiosis skin barrier AD inflammation skin models

TLDR AD-derived keratinocytes effectively mimic inflammation in atopic dermatitis.

This pilot study suggests that keratinocytes (KCs) derived from hair follicles of patients with atopic dermatitis (AD) are a useful model for studying AD-related inflammation. When stimulated with type 2 cytokines IL-4 and IL-13, these AD-derived KCs showed higher expression changes of AD-associated markers compared to healthy KCs. Both 2-dimensional and 3-dimensional skin models using AD-derived KCs demonstrated significant modulation of AD-relevant factors, with IL-13 alone and in combination with IL-4 increasing epidermal spongiosis, a key feature of AD skin. This indicates that AD-derived KCs can effectively mimic AD inflammatory responses in personalized in vitro models.

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