Anti-Inflammatory, Antioxidant, and Antimicrobial Activity of Vitex Negundo and Hibiscus Rosa-Sinensis Herbal Formulation Against Oral Pathogens

The study evaluated the antimicrobial, anti-inflammatory, antioxidant, and cytotoxic activities of a herbal formulation containing Hibiscus rosa-sinensis and Vitex negundo. Both plants have been traditionally used in ethnomedicine for various health conditions. The study employed methods like agar well diffusion for antimicrobial activity and assays such as H2O2, FRAP, and DPPH for antioxidant activity. The formulation demonstrated strong results across all tested activities, indicating its potential in health protection and management of autoimmune diseases. The natural products in the formulation target specific proteins effectively, with fewer side effects and lower toxicity.