Volume Imaging to Interrogate Cancer Cell-Tumor Microenvironment Interactions in Space and Time

The document reviews advancements in high-resolution volume imaging techniques, such as tissue clearing and intravital microscopy (IVM), to study cancer cell interactions with the tumor microenvironment (TME). These techniques enable three-dimensional visualization of tumors, providing insights into the complex architecture and dynamic changes within the TME. The review highlights applications in various cancer models to study cellular interactions, immune cell infiltration, and tumor progression. Volume imaging is presented as a valuable tool for developing cancer therapies, allowing for the evaluation of drug delivery and efficacy. The document underscores the limitations of traditional in vitro assays and the potential of in vivo imaging to improve therapeutic strategies, including immunotherapy.