research Bulge-Derived Epithelial Cells Isolated From Human Hair Follicles Using Enzymatic Digestion Or Explants Result In Comparable Tissue-Engineered Skin
This study developed a protocol for isolating bulge-derived epithelial cells from human hair follicles to create tissue-engineered skin. By using enzymatic digestion, researchers achieved 5.3 times more epithelial cells compared to explant cultures after 8 days, with faster proliferation than a benchmark method. The cells from all methods showed similar morphology and growth rates, and the tissue-engineered skin exhibited comparable differentiation potential. The enzymatic digestion method enhances early-stage cell recovery and expansion while maintaining keratinocyte functionality, providing an efficient technique for tissue engineering and regenerative medicine.
