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GlossaryLiquid Chromatography

technique separates mixture components using liquid and solid phases

Liquid Chromatography (LC) is a laboratory technique used to separate, identify, and quantify components in a mixture. It involves passing a liquid sample through a column packed with a solid adsorbent material, where different substances in the sample move at different speeds, allowing for their separation and analysis. This method is widely used in fields like biochemistry and pharmaceuticals for analyzing complex mixtures, including those related to hair and alopecia research.

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Analysis of Hormone Antagonists in Clinical and Municipal Wastewater by Isotopic Dilution Liquid Chromatography Tandem Mass Spectrometry

research Analysis of hormone antagonists in clinical and municipal wastewater by isotopic dilution liquid chromatography tandem mass spectrometry

51 citations , February 2010 in “Analytical and Bioanalytical Chemistry”

Researchers developed a method to detect hormone-blocking drugs in wastewater and found them in Beijing's sewage, suggesting they can survive sewage treatment.

research Simultaneous pharmacokinetic and pharmacodynamic analysis of 5α-reductase inhibitors and androgens by liquid chromatography tandem mass spectrometry

20 citations , August 2014 in “Talanta”

The method effectively measures drug impact on DHT levels in prostate treatments.

research Studies on the persistence of estradiol benzoate and nortestosterone decanoate in hair of cattle following treatment with growth promoters, determined by ultra-high-performance liquid chromatography–tandem mass spectrometry

18 citations , April 2009 in “Journal of Chromatography A”

Detecting steroid esters in cattle hair is inconsistent and influenced by sampling factors.

research Metabolic study of androsta-1,4,6-triene-3,17-dione in horses using liquid chromatography/high resolution mass spectrometry

17 citations , August 2015 in “Journal of steroid biochemistry and molecular biology/The Journal of steroid biochemistry and molecular biology”

The study found that urine metabolites M1b or M4 are the best indicators of ATD use in horses, with detection possible up to 77 hours in urine and 28 hours in blood.

research Study on dissolution mechanism of cortisol and cortisone from hair matrix with liquid chromatography–tandem mass spectrometry

16 citations , March 2013 in “Clinica Chimica Acta”

Cortisol and cortisone release from hair increases in steps over time during incubation.

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community Liquid Chromatography–Mass Spectrometry (LC-MS) VS Enzyme-Linked Immunosorbent Assay (ELISA)

in Research/Science 4 upvotes 1 year ago

Finasteride and Nizoral shampoo with Ketoconazole are used for hair loss treatment. Liquid Chromatography–Mass Spectrometry (LC-MS) is recommended over Enzyme-Linked Immunosorbent Assay (ELISA) for more accurate DHT testing.

community New and Interesting HairLoss Studies/Papers/Reviews

in Treatment 42 upvotes 6 years ago

Hair loss treatments discussed include Dutasteride with Ketoconazole, tissue engineering strategies, and androgenetic alopecia therapies. Massage doubles follicular retention, improving treatment effectiveness.

community Unexpected Serum DHT Elevation after Generic Dutasteride Use.

in Finasteride/Dutasteride 38 upvotes 1 year ago

Switching from finasteride to a generic dutasteride led to unexpectedly high DHT levels, prompting a switch to a reputed brand and consideration of softgel capsules for better absorption. The user plans to retest DHT levels and may return to finasteride if issues persist.

community Feel like I'm a reasonably good responder

in Is this regrowth? 274 upvotes 1 year ago

The user "OP" reported significant hair regrowth using oral finasteride, oral and topical minoxidil, ketoconazole shampoo, and derma stamping. OP experienced initial shedding but no other side effects.

community Dutasteride SAVES your hair follicles: MAJOR BS on people who say it destroyed their hair permanently.

in Finasteride/Dutasteride 158 upvotes 2 years ago

The conversation discusses the effectiveness of hair loss treatments, specifically finasteride and dutasteride. The conclusion is that dutasteride significantly reduces DHT levels and may be more effective than finasteride for long-term hair retention, with some users reporting personal experiences and side effects.

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