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GlossaryImmunohistochemical Methods

use antibodies to detect specific proteins in tissue samples

Immunohistochemical Methods, often abbreviated as IHC Methods, are laboratory techniques used to visualize specific proteins or antigens in tissue samples. By applying antibodies that bind to these targets and then using a color change or fluorescent marker, scientists and medical professionals can identify the presence and distribution of particular molecules, aiding in disease diagnosis and research.

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research Comparison between the effectiveness of topical Latanoprost and topical Minoxidil in treating Scalp Alopecia Areata in relation to CD8 cell expression: A study using clinical, dermoscopic, and immunohistochemical methods

1 citations , June 2023 in “Jordan Journal of Applied Science - Natural Science Series”

Minoxidil is more effective than Latanoprost for treating patchy alopecia areata.

Immunohistochemical Analysis of Estrogen and Progesterone Receptors in Endometrium and Peritoneal Endometriosis: A New Quantitative Method

research Immunohistochemical analysis of estrogen and progesterone receptors in endometrium and peritoneal endometriosis: A new quantitative method

June 1995 in “International Journal of Gynecology & Obstetrics”

The new method showed that endometriotic tissue has lower estrogen receptor levels but similar progesterone levels compared to normal endometrium, with both following a similar cycle.

research Melanocyte Subpopulation Turnover During the Human Hair Cycle: An Immunohistochemical Study

86 citations , August 2000 in “Pigment cell research”

Melanocyte activity in hair follicles is linked to the hair growth cycle, being active in growth phases and inactive in rest phases.

research Parathyroid Hormone-related Protein in Normal and Neoplastic Canine Tissues: Immunohistochemical Localization and Biochemical Extraction

54 citations , May 1994 in “Veterinary Pathology”

PTHrP is higher in certain dog tumors and may act as a local growth factor.

research Immunohistochemical demonstration of keratins 8 and 14 in benign tumours of the skin appendage

38 citations , November 1991 in “Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin”

Keratins 8 and 14 can help identify and diagnose benign skin tumors.

research IMMUNOHISTOCHEMICAL EXPRESSION OF KERATIN PROTEINS IN KERATOACANTHOMAS OF THE SKIN

2 citations , March 1994 in “Oncology Reports”

Keratoacanthomas and squamous cell carcinomas have similar keratin patterns, making them hard to tell apart.

research Methods in hair research: how to objectively distinguish between anagen and catagen in human hair follicle organ culture

132 citations , September 2009 in “Experimental Dermatology”

A reliable system was developed to distinguish hair growth stages, aiding in identifying hair growth promoters or inhibitors.

research Tenascin expression in normal human adult skin and skin appendage tumours

17 citations , June 1994 in “Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin”

Tenascin is present in normal skin and certain skin tumors but not in sebaceous glands or their tumors.

research [Peptidergic innervation in the sinus hair follicles of several mammalian species].

7 citations , October 1992 in “PubMed”

Sinus hair follicles in mammals have different nerve fiber types with species-specific patterns, especially in cats.

research Runx1 transcription factor is involved in the regulation of KAP5 gene expression in human hair follicles

6 citations , January 2006 in “Journal of dermatological science”

Runx1 helps control the KAP5 gene in human hair follicles.

research Telogen elongation in the hair cycle of ob/ob mice

5 citations , August 2015 in “Bioscience, Biotechnology, and Biochemistry”

Obese mice with a leptin gene mutation have a longer resting phase in their hair cycle, which may help understand certain hair loss conditions.

research Study on VEGF in the Expression of Skin Hair Follicle and the Relationship with Microvessel Density by Liaoning Cashmere Goat in Fetal Period

1 citations , January 2013 in “China Animal Husbandry & Veterinary Medicine”

VEGF increases in goat hair follicles during fetal development, boosting skin blood vessel density.

research Milia may originate from the outermost layers of the hair bulge of the outer root sheath: A case report

November 2016 in “Oncology Letters”

Milia may come from the outer part of the hair follicle.

research A practical guide for the study of human and murine sebaceous glands in situ

56 citations , September 2013 in “Experimental Dermatology”

The guide explains how to study human and mouse sebaceous glands using various staining and imaging techniques, and emphasizes the need for standardized assessment methods.

research ABO Blood Grouping of Non-medullated Scalp Hair Using Immunohistochemical Staining

January 2010 in “Nihon Hou Kagaku Gijutsu Gakkaishi”

The modified staining method can determine the ABO blood group of hair samples accurately.

research Immunohistochemical Analysis of the Mechanistic Target of Rapamycin and Hypoxia Signalling Pathways in Basal Cell Carcinoma and Trichoepithelioma

15 citations , September 2014 in “PloS one”

The study found that analyzing certain cell signaling pathways is not a reliable method to tell apart two types of skin tumors.

Applying FACS-Based Single Cell RNA-Seq to Study Neonatal Mouse Skin

research 21: NOVEL USE OF MATRIX FOR HAIR GROWTH

April 2010 in “Plastic and Reconstructive Surgery”

The technique can potentially treat hair loss by using a matrix to grow new hair from cells.

research Characteristic intraepidermal nerve fibre endings of the intervibrissal fur in the mystacial pad of the rat: morphological details revealed by intravital methylene blue staining and the zinc iodide-osmium tetroxide technique

5 citations , July 1999 in “Journal of Anatomy”

Methylene blue staining effectively highlights detailed nerve structures in rat fur.

research Isolation and cultivation of the cells of hair follicles in vitro,and observation of the biological characteristics of them.

1 citations , January 2009 in “Journal of Dermatology and Venereology”

Cells from the upper hair follicle grow more actively, suggesting stem cells may be located there.

research Differentiation and Apoptosis in Pilomatrixoma

9 citations , January 2011 in “American Journal of Dermatopathology”

Pilomatrixoma involves abnormal hair keratin production and cell death, causing debris and cysts.

research Characteristic intraepidermal nerve fibre endings of the intervibrissal fur in the mystacial pad of the rat: morphological details revealed by intravital methylene blue staining and the zinc iodide‐osmium tetroxide technique