Bioprinted Recombinant Human-Collagen-Based In Vitro Skin Models for Assessing Effects of Nano-ZnO on Dermis

In this study, researchers developed a biomimetic 3D skin model using methacrylated recombinant human collagen bioinks to assess the effects of nano-zinc oxide (nano-ZnO) on skin. The model, which mimics the skin of children and adults, includes dermal layers with human skin fibroblasts and human umbilical vein endothelial cells, as well as epidermal layers with keratinocytes. It successfully replicates native skin architecture and allows for the evaluation of key markers. The study found that nano-ZnO significantly affects genes related to apoptosis, inflammation, and oxidative stress in skin cells, highlighting its potential impact on skin health.