Cell-Free Fat Extract Combined with GelMA Hydrogel to Improve Survival Rate of Random Skin Flaps in Mice

This study investigates the use of cell-free fat extract (Ceffe) encapsulated in gelatin methacrylate (GelMA) hydrogel to improve the survival rate of random skin flaps in mice. Ceffe, known for its bioactive compounds, was limited by quick leakage and short effectiveness when injected directly. By encapsulating Ceffe in GelMA, the study developed a stable release system that promotes angiogenesis and flap repair, as confirmed by various assays and histological analyses. The Ceffe@GelMA system demonstrated significant pro-repair activity, enhanced pro-angiogenic potential, and favorable biocompatibility, suggesting its therapeutic efficacy in preventing flap necrosis and representing a significant advancement in clinical treatment strategies.