Cell-Free Fat Extract Combined with GelMA Hydrogel to Improve Survival Rate of Random Skin Flaps in Mice

This study explores the use of Cell-Free Fat Extract (Ceffe) combined with GelMA hydrogel to improve the survival rate of random skin flaps in 30 male C57BL/6 mice. The combination enhances flap survival by promoting angiogenesis, reducing oxidative stress, and maintaining stable Ceffe concentrations through a sustained-release system. The Ce@GM hydrogel demonstrated significant improvements in microvessel density and reduced flap necrosis to 23.4% compared to 96% in the control group. The findings suggest that this approach could be a promising candidate for clinical applications in flap transplantation and reconstructive surgeries, although further research is needed to confirm its efficacy in humans.