Comparative Analysis of Wharton's Jelly Mesenchymal Stem Cell Isolated Using Explant and Enzymatic Methods

    November 2019 in “ Journal of Physics Conference Series ”
    Wahyu Widowati, Rimonta F. Gunanegara, Rizal Rizal, Wahyu Setia Widodo, Annisa Amalia, Satrio Wibowo, Kusworini Handono, M Marlina, I Nyoman Ehrich Lister, Linda Chiuman
    TLDR Both methods effectively isolate high-quality stem cells from umbilical cords.
    The study compared two methods for isolating human Wharton’s Jelly Mesenchymal Stem Cells (hWJ-MSCs) from umbilical cords: the explant method and the enzymatic method. Both methods successfully isolated hWJ-MSCs, which showed high purity and positive expression of markers CD44, CD90, CD105, and CD73. The isolated cells demonstrated the ability to differentiate into adipogenic, chondrogenic, and osteogenic lineages, indicating their potential for use in regenerative medicine. Both methods were found to be efficient in producing hWJ-MSCs with high proliferation capacity.
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