Establishment and Characterization of Immortalized Human Dermal Papilla Cells Expressing Human Papillomavirus 16 E6/E7

November 2023 in “ Journal of Microbiology and Biotechnology ”

Seonhwa Kim, Kyeong-Bae Jeon, Hyo‐Min Park, Jinju Kim, Chae-Min Lim, Do‐Young Yoon

human dermal papilla cells HPV16 E6/E7 p53 pRb CDK inhibitor p21 CDK2 cyclin E E2F family genes Wnt/β-catenin pathway hair follicles human papillomavirus 16 Wnt pathway

The study successfully established immortalized human dermal papilla cells (HDPCs) by transfecting them with human papillomavirus 16 (HPV16) E6/E7 oncogenes, overcoming the limitations of primary HDPCs such as reduced proliferative capacity and hair induction potential. These immortalized cells demonstrated higher proliferative activity, with downregulated p53 and pRb proteins and decreased expression of the CDK inhibitor p21. Additionally, cell cycle-related genes and proteins, including CDK2, cyclin E, and E2F family genes, were upregulated. Importantly, the immortalized HDPCs retained their ability to respond to the Wnt/β-catenin pathway and form hair follicles, making them valuable for in vitro hair growth and regeneration studies.

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