Establishment and Molecular Characterization of Human Dermal Mesenchymal-Like Stem Cells Derived from Human Scalp Biopsy of Androgenetic Alopecia Patient

The study established a human dermal mesenchymal-like stem cell line from a scalp biopsy of an androgenetic alopecia patient. These cells, termed "Human Dermal Mesenchymal-like Stem Cells (hDMSCs)," were derived from the bulge region of hair follicles and exhibited both epithelial and mesenchymal characteristics, expressing markers such as CD34, Keratin 18, Oct4, Nanog, and SOX2. The optimal growth conditions for these cells were found to be at a 3% serum concentration. The hDMSCs were maintained up to passage 10 and were proposed as an in vitro model to study the mechanisms of alopecia and related skin disorders.