JMJD3 and NF-κB-Dependent Activation of Notch1 Gene Is Required for Keratinocyte Migration During Skin Wound Healing

The study demonstrated that the JMJD3 and NF-κB-dependent activation of the Notch1 gene was crucial for keratinocyte migration during skin wound healing. Researchers identified Notch1 as a direct target of JMJD3 and NF-κB in wounded keratinocytes, with its up-regulation at the wound edge being essential for the process. Notch1 was found to activate RhoU and PLAU genes, which are important for cell migration. Inactivation of Notch1 led to decreased filopodia formation and increased focal adhesion and actin stress fiber, resulting in reduced keratinocyte migration and impaired wound healing. This highlighted the significance of the JMJD3/NF-κB-Notch pathway in the molecular mechanism of keratinocyte wound healing.