Label-Free Monitoring of Endometrial Cancer Progression Using Multiphoton Microscopy

This study explores the use of multiphoton microscopy (MPM) as a label-free method to monitor endometrial cancer progression by analyzing collagen, which is often overlooked in current diagnostic protocols. MPM, utilizing second-harmonic generation and two-photon excited fluorescence, allows visualization of the endometrial microenvironment and morphological changes in glands during cancer development. Key features identified include irregular gland structures, increased gland-to-interstitium ratios, and atypical glandular epithelial cells. The study highlights that endometrial cancer formation is marked by changes in collagen fiber length, width, and arrangement. This method shows promise as an effective tool for early diagnosis and detection of malignant lesions in endometrial cancer.