TLDR Multiphoton microscopy can effectively image live cells in cornea, skin, and hair follicles over time.
The study demonstrated the use of multiphoton microscopy for long-term imaging of cells in live animals, specifically focusing on the cornea, skin, and hair follicles in transgenic mice. This technique allowed for cell imaging and tracing at a single-cell resolution, utilizing fluorescent proteins to tag specific cell populations. The method provided insights into cell dynamics and structural information from the extracellular matrix, offering advantages over conventional histological analysis in stem cell research.
56 citations
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June 2015 in “Nature Protocols” Two-photon microscopy helps observe hair follicle stem cell behaviors in mice.
305 citations
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June 2012 in “Nature” Hair regeneration needs dynamic cell behavior and mesenchyme presence for stem cell activation.
417 citations
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September 2005 in “PLoS biology” Understanding gene expression in hair follicles can reveal insights into hair growth and disorders.
79 citations
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November 2016 in “EMBO Reports” Disruptions in mammary stem cell division can lead to cancer, but targeting these processes might help treat breast cancer.
58 citations
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November 2013 in “Journal of Innovative Optical Health Sciences” Multiphoton microscopy is a promising tool for detailed skin imaging and could improve patient care if its challenges are addressed.
16 citations
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September 2016 in “Experimental Dermatology” Two-photon microscopy effectively tracks live stem cell activity in mouse skin with minimal harm and clear images.
5 citations
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May 2023 in “Frontiers in Immunology” Advanced imaging methods have improved understanding of cancer cell interactions and treatment strategies.
97 citations
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December 2021 in “Cells” Designing effective fluorescence microscopy experiments requires careful consideration of hardware, biological models, and imaging agents.
