Genomic and Functional Characterization of a Novel Halophilic Bacteriophage Targeting Carbapenem-Resistant Klebsiella Pneumoniae

This study presents the isolation and characterization of a novel halophilic lytic bacteriophage, Halo KS-7, which targets carbapenem-resistant Klebsiella pneumoniae (CRKP), a multidrug-resistant pathogen. Halo KS-7, classified as a Myoviridae bacteriophage, demonstrated strong lytic activity against CRKP, optimal at 37 °C and neutral pH, with effective activity across pH 4-10 and in high-salinity conditions. In vitro assays showed a short latent period and sustained suppression of K. pneumoniae growth, while in vivo tests in BALB/c mice indicated significant wound healing and infection control. Genomic analysis revealed a 58.716 kb linear dsDNA genome with 49 predicted ORFs, lacking genes for lysogeny or antibiotic resistance, and containing auxiliary factors that enhance bacterial killing. The study highlights Halo KS-7's novelty, antibacterial efficacy, and potential as a biocontrol agent against CRKP, supporting its clinical development for managing multidrug-resistant infections.