Optimization of Culture Conditions for Expansion of Human Epidermal Stem Cells

The study optimized in vitro culture methods for human epidermal stem cells (EpSCs) using different media and supplements. Explant culture in MEM-α with 20% FBS initially showed promising cell attachment but lost morphology by passage 1, while suspension culture with MEM-α and growth factors yielded more EpSCs. EpiLife media with KGF, EGF, and HKGS supported proper EpSC attachment and morphology. EpSCs cultured in MEM-α and EpiLife with growth factors demonstrated high proliferation and clonogenic potential, expressing markers like p63 and KRT5. These findings suggest that EpSCs cultured under these conditions are suitable for skin regenerative medicine.