Optimization of Gelatin and Crosslinker Concentrations in a Gelatin/Alginate-Based Bioink with Potential Applications in a Simplified Skin Model

This study optimized a gelatin/alginate-based bioink for 3D bioprinting a simplified skin model by testing various gelatin (10%, 15%, 20%) and CaCl2 concentrations (100, 150, 200 mM). The optimal formulation, GEL-15-ALG with 15% gelatin and 150 mM CaCl2, demonstrated superior printability, biodegradation, and swelling properties. It supported the viability and proliferation of human fibroblasts and keratinocytes for up to 14 days, suggesting potential applications in drug evaluation and cosmetic testing as an alternative to animal models. The study highlights the need for further research to enhance cell proliferation and tissue maturation.