Chemical Constituents from Osmanthus Fragrans Var. Aurantiacus Makino with Their In Vitro and In Silico Studies Targeting Anti-Inflammation by Suppressing ERK 1/2 MAPK Signaling

The study on Osmanthus fragrans var. aurantiacus Makino revealed its potential as a therapeutic agent for inflammatory bowel diseases due to its antioxidative and anti-inflammatory properties. Researchers identified 18 compounds, including a new one, using GNPS-FBMN and in vitro assays. These compounds were shown to inhibit inflammation by targeting COX-2 and iNOS enzymes and blocking ERK 1/2 MAPK signaling, as confirmed by Western blotting. In silico studies further supported the compounds' binding affinity and dynamic features, suggesting their potential use as phytochemicals for inflammation treatment.