Effects of Repeated Exposure to Ozonated Water on Hair Fiber Structure and Integrity: Part I

This study examined the effects of repeated exposure to ozonated water on hair fibre structure and integrity. The results showed that ozonated water caused oxidative modifications in hair proteins, lipids, and pigments, leading to changes in structural, chemical, and functional properties. Specifically, there were increases in cysteic acid-related bands and carbonyl groups, indicating protein and lipid oxidation. Morphological changes included cuticle fusion and increased cortical fluorescence. Mechanical properties were affected, with increased denaturation temperature and post-yield stiffness, suggesting altered crosslink density and keratin disruption. Despite these changes, alterations in shine and colour were minimal. The study concludes that ozone exposure results in a unique damage signature affecting both the cuticle and cortex, warranting further investigation into lipidomic and biochemical changes and the hair's response to additional stressors.