Accelerative Action of Topical Piperonylic Acid on Mice Full Thickness Wound by Modulating Inflammation and Collagen Deposition

The study investigated the effects of topical piperonylic acid on wound healing in C57BL6/J male mice with 6 mm dorsal skin wounds. Piperonylic acid, a metabolite found in peppers, was shown to enhance wound healing from day 6 post-injury until closure by activating the epidermal growth factor receptor (EGFR). This activation led to the modulation of gene expression related to inflammation and healing, including interleukin-6, il-1β, tumor necrosis factor-α, il-10, monocyte chemoattractant protein-1, and insulin-like growth factor-1. By day 19, treated wounds exhibited greater type I collagen deposition and a morphology resembling intact skin, with more dermal papillae and hair follicles. The findings suggested that piperonylic acid could be a promising treatment for skin wounds.