A Rapid Screening LC-MS/MS Method Based on Conventional HPLC Pumps for the Analysis of Low Molecular Weight Xenobiotics: Application to Doping Control Analysis
July 2010
in “
Drug Testing and Analysis
”
TLDR A quick method was developed to detect banned substances in urine for doping control.
The study developed a rapid multi-analyte screening method for detecting low molecular weight xenobiotics in urine, specifically for doping control. The method utilized conventional HPLC pumps and achieved chromatographic separation in 7.2 minutes using a reversed-phase 2.7 µm fused-core particle column. This approach was tested on 20 blank urine samples spiked with 45 prohibited compounds, including diuretics, glucocorticoids, stimulants, anti-oestrogens, and others. The method demonstrated specificity with no significant interference, and all analytes were distinguishable with detection limits between 5 ng/mL and 350 ng/mL, below the WADA's Minimum Required Performance Levels. The study concluded that the method was effective for separating compounds, including those with similar retention times and fragmentation patterns.
