Defining a Region of the Human Keratin 6a Gene That Confers Inducible Expression in Stratified Epithelia of Transgenic Mice

The study identified a specific region in the human keratin 6a (K6a) gene necessary for inducible expression in stratified epithelia of transgenic mice, particularly following injury or chemical treatment. Researchers found that the proximal 960 base pairs of the 5′-upstream sequence were crucial for rapid induction, with regulatory sequences located between −960 and −550 base pairs. The study demonstrated that β-galactosidase activity was induced in keratinocytes near wound edges, with the strongest response in KT1 transgenic lines. Chemical agents like PMA and RA also induced expression, with RA having a stronger effect. However, the 5.2 kb upstream sequence was insufficient for sustained expression in chronic conditions, indicating the need for additional regulatory elements. The findings highlighted the unique regulatory organization of the K6a gene and provided insights into gene expression control in wound repair and other conditions affecting stratified epithelia.