Author Response: Single-Cell RNA and TCR Sequencing Reveals the Proportion and Characteristics of Dual TCR Regulatory T Cells in Mouse Lymphoid and Non-Lymphoid Tissues
October 2025
TLDR 10-20% of mouse Tregs have dual TCRs, suggesting potential functional benefits.
The study by Xu and Peng et al. explores dual TCR expression in FoxP3+ regulatory CD4+ T cells (Tregs) using single-cell TCR sequencing, revealing that 10-20% of Tregs in mouse tissues express dual TCRs. While the study suggests potential functional benefits of dual TCR expression, it is limited by the lack of functional validation and comprehensive gene expression analysis. The research identifies significant proportions of dual TCR Tregs and highlights their potential role in complex functional regulation, but acknowledges the need for further experiments to validate findings at the protein level and address methodological weaknesses. Despite these limitations, the study provides a foundation for future research on dual TCR Treg functions.
