Single-Cell Proteomics Uncovers Dual Traits of Dermal Sheath Cells in Wound Repair

This study investigates the role of dermal sheath cells (DSCs) in wound healing using single-cell proteomics in a mouse model. DSCs, located around hair follicles, exhibit dual epithelial and mesenchymal traits, contributing to all wound healing phases: inflammation, proliferation, and remodeling. Key proteins such as keratins and collagens are involved in these processes. Temporal clustering reveals functional shifts, with early phases linked to metabolic activation and later phases to extracellular matrix remodeling. The study identifies pathways like interferon-gamma response and core genes like Samhd1 and Psma3, crucial for immune regulation and inflammation resolution. The findings highlight DSCs' potential in enhancing wound healing and preventing fibrosis, offering insights for therapeutic development.