Tissue Harvesting HuBMAP JHU-TMC Version 1

    March 2024
    Kyu Sang Han, Pei-Hsun Wu, Joel Sunshine, Ashley Kiemen, Sashank Reddy, Denis Wirtz
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    TLDR Quickly fix tissue in formalin after excision to preserve it.
    The document outlines a protocol for harvesting human tissue biopsies and preparing them for histological processes. It involves using prefilled histology containers with 10% Neutral Buffered Formalin (NBF) to fix the tissue immediately after excision to prevent degradation. The protocol specifies the preparation of the formalin solution and emphasizes the importance of minimizing the time delay between tissue excision and fixation, known as warm ischemic time, to maintain tissue integrity. The protocol excludes areas with a paucity of hair follicles, such as those affected by advanced androgenic alopecia or alopecia areata.
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