TMT-Based Quantitative Proteomics Reveals the Genetic Mechanisms of Secondary Hair Follicle Development in Fine-Wool Sheep
February 2025
in “
PLoS ONE
”
secondary hair follicles fine-wool sheep TMT-based quantitative proteomics fetal development skin tissue protein profiles differentially expressed proteins focal adhesion ECM-receptor interaction estrogen signaling pathways COL1A1 THBS3 ITGA6 COL6A1 THBS4 hair follicles wool quality proteomics protein profiles estrogen pathways
TLDR Key proteins influence wool quality by affecting hair follicle development in sheep.
This study investigates the protein-mediated mechanisms involved in the fetal development of secondary hair follicles in fine-wool sheep, which are crucial for wool quality. Using TMT-based quantitative proteomics, researchers analyzed skin tissue protein profiles at four fetal development stages (75, 85, 95, and 105 gestational days). They observed an increase in the density and ratio of secondary to primary hair follicles from 85 to 105 days. Bioinformatic analysis revealed 238, 35, and 348 differentially expressed proteins across the developmental stages, highlighting the roles of focal adhesion, ECM-receptor interaction, and estrogen signaling pathways. Key proteins such as COL1A1, THBS3, ITGA6, COL6A1, and THBS4 were identified as potential candidates in secondary hair follicle initiation, enhancing the understanding of molecular mechanisms affecting wool quality.
