Cutaneous TRPV4 Potentiates the Afferent Transmission of Electroacupuncture Stimulation

The study explores the role of TRPV4 channels in modulating sensory neuron excitability during electroacupuncture stimulation in mice. Using in vivo calcium imaging, researchers found that higher-intensity electroacupuncture activates more sensory neurons, particularly those associated with Aδ and Aβ fibers. TRPV4, expressed in non-neuronal skin cells, enhances neuronal responses to stimulation through neuroimmune interactions, likely involving macrophage-derived mediators. Pharmacological activation of TRPV4 increased calcium activity in sensory neurons, suggesting its potential role in acupuncture signal transduction and therapeutic effects. These findings highlight TRPV4 as a potential target for enhancing acupuncture efficacy.