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This case-control study investigated the association between non-scarring alopecia and vitamin D levels in 69 patients with alopecia and 69 age-matched controls. The study found that more than half of both groups had vitamin D deficiency, and nearly one-third had vitamin D insufficiency. Although the mean vitamin D level was slightly lower in the alopecia group (23.12±16.70) compared to the controls (23.75±18.63), the difference was not statistically significant (p=0.06). The study concludes that vitamin D deficiency is frequently associated with non-scarring alopecia, suggesting that vitamin D levels should be routinely measured in patients with hair loss. Additionally, due to the comparable prevalence of vitamin D insufficiency in the control group, the study recommends community-level food fortification with vitamin D.